PhD project: Isolation of RG-I oligosaccharides for monoclonal antibody production and antibody use as detection tools for novel chromatographic analyses of RG-I

Research interests: Cell walls, monoclonal antibodies, pectin, Immunoassay, xylan, glycan profiling.

Organisation: University of Leeds

Contact: bsvrc(at)leeds.ac.uk

LinkedIn account: www.linkedin.com/pub/valerie-cornuault

ResearchGate account: www.researchgate.net/profile/Valerie_Cornuault

Thesis

Molecular and biochemical tools for plant cell wall glycan analysis. Defended on 12 December 2014.

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Scientific papers in peer-reviewed journals

V. Cornuault^a, F. Buffetto^b, M.G. Rydahl^c, S.E. Marcus^a, T.A. Torode^a, J. Xue^a, M.-J. Crépeau^b, N. Faria-Blanc^d, W.G.T. Willats^c, P. Dupree^d, M.-C. Ralet^b, J.P. Knox^a (^aU.Leeds, ^bINRA, ^cU.Copenhagen, ^dU.Cambridge). Monoclonal antibodies indicate low-abundance links between heteroxylan and other glycans of plant cell walls. Planta, Vol. 242, pp. 1321-1334 (2015)

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F. Buffetto^a, V. Cornuault^b, M.G. Rydahl^c, D. Ropartz^a, C. Alvarado^a, V. Echasserieau^a, S. Le Gall^a, B. Bouchet^a, O. Tranquet^a, Y. Verhertbruggen^a, W.G.T. Willats^c, J.P. Knox^b, M.-C Ralet^a, F. Guillon^a (^aINRA, ^bU.Leeds, ^cU.Copenhagen). The deconstruction of pectic rhamnogalacturonan I unmasks the occurrence of a novel arabinogalactan oligosaccharide epitope. Plant and Cell Physiology, Vol. 56, issue 11, pp. 2181-2196 (2015)

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Richard Palmer^a, Valérie Cornuault^b, Susan E. Marcus^b, J. Paul Knox^b, Peter R. Shewry^a, Paola Tosi^a  (^aRothamsted Research, ^bU.Leeds) Comparative in situ analyses of cell wall matrix polysaccharide dynamics in developing rice and wheat grain. Planta, vol. 241, issue 3, pp 669-685 (2015)

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V. Cornuault, J.P. Knox (U.Leeds) Sandwich Enzyme-linked Immunosorbent Assay (ELISA) Analysis of Plant Cell Wall Glycan Connections. http://bio-protocol.org/wenzhang.aspx?id=1106#.U5HFQy9JmRt

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V. Cornuault^a, I.W. Manfield^a, M-C. Ralet^b and J.P. Knox^a (^aU.Leeds, ^bINRA) Epitope detection chromatography: a method to dissect the structural heterogeneity and inter-connections of plant cell-wall matrix glycans. The Plant Journal 78, pp. 715–722 (2014)

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K.J.D. Lee^a, V. Cornuault^a, I.W. Manfield^a, M-C. Ralet^b and J.P. Knox^a (^aU.Leeds, ^bINRA). Multi-scale spatial heterogeneity of pectic rhamnogalacturonan I (RG–I) structural features in tobacco seed endosperm cell walls. The plant journal, 75, pp.1018-1027 (2013)

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Oral Presentations

V. Cornuault (U.Leeds). Epitope Detection Chromatography: a new method to study pectin heterogeneity. Pacifichem on 15-20 December 2015 in Honolulu (USA).

F. Buffetto^a & V. Cornuault^b, M. Gro Rydahl^c, M.-J. Crépeau^a, O. Tranquet^a, W.G.T. Willat^c, P. Knox^b, F. Guillon^a, M.-C.  Ralet^a (^aINRA, ^bU.Leeds, ^cU.Copenhagen). Un nouvel anticorps reconnaissant le domaine pectique. Presented at the Congress of the French cell wall network on July, 7-9, 2014 in Amiens (FR).V. Cornuault^a, F. Buffetto^b, I. Manfield^a, M.-C. Ralet^b, J.P. Knox^a (^aU. Leeds, ^bINRA) Molecular and biochemical tools for the analysis of glycans in plant cell walls. Presented at WallTraC symposium in May 2014 in Paris (FR).F. Buffetto^a & V. Cornuault^b, M. Gro Rydahl^c, M.-J. Crépeau^a, O. Tranquet^a, W.G.T. Willat^c, P. Knox^b, F. Guillon^a, M.-C.  Ralet^a (^aINRA, ^bU.Leeds, ^cU.Copenhagen). Production and fine characterization of new antibodies against rhamnogalacturonan I. Presented at WallTraC symposium in May 2014 in Paris (FR).V. Cornuault^a, F. Buffetto^b, K.J.D. Lee^a, M.-J. Crépeau^b, M.-C.  Ralet^b, J.P. Knox^a (^aU.Leeds, ^bINRA). Development of Epitope Detection Chromatography for RG-I analysis. Presented at the Gordon Research Seminar in August 2012 in Boston (USA) - Presentation not publicly available.

Posters

V. Cornuault^a, I. Manfield^a, M.-C. Ralet^b, J.P. Knox^a (^aU.Leeds, ^bINRA). Dissecting the complexity of cell wall matrix polysaccharides using epitope detection chromatography. Presented at the 5th International Conference on Biosynthesis of Plant Cell Wall on July, 27-31, 2014 in Palm Cove (AU)

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V. Cornuault^a, I. Manfield^a, J.P. Knox^a (^aU.Leeds). Epitope Detection Chromatography (EDC): methodology to dissect the complexity of plant cell walls. Presented at at the XIIIth Cell Wall Meeting on July, 7-12, 2013 in Nantes (FR).

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F.Buffetto^a,V. Cornuault^b, M.G. Rydal^c, M.-J. Crépeau^a, O. Tranquet^a , W. G. T. Willats^c, J.P. Knox^b, F. Guillon^a, M.-C. Ralet^a (^aINRA, ^bU.Leeds, ^cU.Copenhagen). Production and fine characterization of new monoclonal antibodies against RGI. Presented at at the XIIIth Cell Wall Meeting on July, 7-12, 2013 in Nantes (FR).

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V. Cornuault^a, I. Manfield^a, J.P. Knox^a (^aU.Leeds). Epitope Detection Chromatography for plant cell walls. Presented at the 2nd Plants and People Conference on June, 18-19, 2013 in Potsdam (DE).

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V. Cornuault^a, F. Buffetto^b, K.J.D. Lee^a, M.-J. Crépeau^b, M.-C. Ralet^b, J.P. Knox^a (^aU.Leeds, ^bINRA). Isolation of RG-I oligosaccharides for monoclonal antibody production and antibody use as detection tools for novel chromatographic analyses of RG-I diversity. Presented at the Gordon conference on Plant Cell Wall Research on August 5-10, 2012 in Waterville (USA) - Poster not publicly available.F.Buffetto^a,V. Cornuault^b, M.-J. Crépeau^a, O. Tranquet^a, P. Knox^b, F. Guillon^a, M.-C. Ralet^a (^aINRA, ^bU.Leeds). Production of low branched rhamnogalacturonan I oligosaccharides from sugar beet pulp. Presented at the Summer Course Glycoscience on June 3-7, 2012 in Gorningen (NL) and at the Plant and Seaweed Polysaccharides Symposium on July 17-20, 2012 in Nantes (FR).

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F.Buffetto^a,V. Cornuault^b, M.-J. Crépeau^a, O. Tranquet^a, P. Knox^b, F. Guillon^a, M.-C. Ralet^a (^aINRA, ^bU.Leeds). Production of low branched rhamnogalacturonan I oligosaccharides from sugar beet pulp. Presented at the Summer Course Glycoscience on June 3-7, 2012 in Gorningen (NL) and at the Plant and Seaweed Polysaccharides Symposium on July 17-20, 2012 in Nantes (FR).

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