PhD project: Isolation of RG-I oligosaccharides for monoclonal antibody production and antibody use as detection tools for novel chromatographic analyses of RG-I
Research interests: Cell walls, monoclonal antibodies, pectin, Immunoassay, xylan, glycan profiling.
Organisation: University of Leeds
Contact: bsvrc(at)leeds.ac.uk
LinkedIn account: www.linkedin.com/pub/valerie-cornuault
ResearchGate account: www.researchgate.net/profile/Valerie_Cornuault
Thesis
Molecular and biochemical tools for plant cell wall glycan analysis. Defended on 12 December 2014.
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Scientific papers in peer-reviewed journals
V. Cornuaulta, F. Buffettob, M.G. Rydahlc, S.E. Marcusa, T.A. Torodea, J. Xuea, M.-J. Crépeaub, N. Faria-Blancd, W.G.T. Willatsc, P. Dupreed, M.-C. Raletb, J.P. Knoxa (aU.Leeds, bINRA, cU.Copenhagen, dU.Cambridge). Monoclonal antibodies indicate low-abundance links between heteroxylan and other glycans of plant cell walls. Planta, Vol. 242, pp. 1321-1334 (2015)
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F. Buffettoa, V. Cornuaultb, M.G. Rydahlc, D. Ropartza, C. Alvaradoa, V. Echasserieaua, S. Le Galla, B. Boucheta, O. Tranqueta, Y. Verhertbruggena, W.G.T. Willatsc, J.P. Knoxb, M.-C Raleta, F. Guillona (aINRA, bU.Leeds, cU.Copenhagen). The deconstruction of pectic rhamnogalacturonan I unmasks the occurrence of a novel arabinogalactan oligosaccharide epitope. Plant and Cell Physiology, Vol. 56, issue 11, pp. 2181-2196 (2015)
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Richard Palmera, Valérie Cornuaultb, Susan E. Marcusb, J. Paul Knoxb, Peter R. Shewrya, Paola Tosia (aRothamsted Research, bU.Leeds) Comparative in situ analyses of cell wall matrix polysaccharide dynamics in developing rice and wheat grain. Planta, vol. 241, issue 3, pp 669-685 (2015)
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V. Cornuault, J.P. Knox (U.Leeds) Sandwich Enzyme-linked Immunosorbent Assay (ELISA) Analysis of Plant Cell Wall Glycan Connections. http://bio-protocol.org/wenzhang.aspx?id=1106#.U5HFQy9JmRt
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V. Cornuaulta, I.W. Manfielda, M-C. Raletb and J.P. Knoxa (aU.Leeds, bINRA) Epitope detection chromatography: a method to dissect the structural heterogeneity and inter-connections of plant cell-wall matrix glycans. The Plant Journal 78, pp. 715–722 (2014)
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K.J.D. Leea, V. Cornuaulta, I.W. Manfielda, M-C. Raletb and J.P. Knoxa (aU.Leeds, bINRA). Multi-scale spatial heterogeneity of pectic rhamnogalacturonan I (RG–I) structural features in tobacco seed endosperm cell walls. The plant journal, 75, pp.1018-1027 (2013)
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Oral Presentations
V. Cornuault (U.Leeds). Epitope Detection Chromatography: a new method to study pectin heterogeneity. Pacifichem on 15-20 December 2015 in Honolulu (USA).
F. Buffettoa &
V. Cornuaultb, M. Gro Rydahl
c, M.-J. Crépeau
a, O. Tranquet
a, W.G.T. Willat
c, P. Knox
b, F. Guillon
a, M.-C. Ralet
a (
aINRA,
bU.Leeds,
cU.Copenhagen). Un nouvel anticorps reconnaissant le domaine pectique. Presented at the Congress of the French cell wall network on July, 7-9, 2014 in Amiens (FR).
V. Cornuaulta, F. Buffetto
b, I. Manfield
a, M.-C. Ralet
b, J.P. Knox
a (
aU. Leeds,
bINRA) Molecular and biochemical tools for the analysis of glycans in plant cell walls. Presented at WallTraC symposium in May 2014 in Paris (FR).
F. Buffettoa &
V. Cornuaultb, M. Gro Rydahl
c, M.-J. Crépeau
a, O. Tranquet
a, W.G.T. Willat
c, P. Knox
b, F. Guillon
a, M.-C. Ralet
a (
aINRA,
bU.Leeds,
cU.Copenhagen). Production and fine characterization of new antibodies against rhamnogalacturonan I. Presented at WallTraC symposium in May 2014 in Paris (FR).
V. Cornuaulta, F. Buffetto
b, K.J.D. Lee
a, M.-J. Crépeau
b, M.-C. Ralet
b, J.P. Knox
a (
aU.Leeds,
bINRA). Development of Epitope Detection Chromatography for RG-I analysis. Presented at the Gordon Research Seminar in August 2012 in Boston (USA) - Presentation not publicly available.
Posters
V. Cornuaulta, I. Manfield
a, M.-C. Ralet
b, J.P. Knox
a (
aU.Leeds,
bINRA). Dissecting the complexity of cell wall matrix polysaccharides using epitope detection chromatography. Presented at the 5th International Conference on Biosynthesis of Plant Cell Wall on July, 27-31, 2014 in Palm Cove (AU)
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V. Cornuaulta, I. Manfield
a, J.P. Knox
a (
aU.Leeds). Epitope Detection Chromatography (EDC): methodology to dissect the complexity of plant cell walls. Presented at at the XIIIth Cell Wall Meeting on July, 7-12, 2013 in Nantes (FR).
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F.Buffettoa,
V. Cornuaultb, M.G. Rydal
c, M.-J. Crépeau
a, O. Tranquet
a , W. G. T. Willats
c, J.P. Knox
b, F. Guillon
a, M.-C. Ralet
a (
aINRA,
bU.Leeds,
cU.Copenhagen). Production and fine characterization of new monoclonal antibodies against RGI. Presented at at the XIIIth Cell Wall Meeting on July, 7-12, 2013 in Nantes (FR).
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V. Cornuaulta, I. Manfield
a, J.P. Knox
a (
aU.Leeds). Epitope Detection Chromatography for plant cell walls. Presented at the 2nd Plants and People Conference on June, 18-19, 2013 in Potsdam (DE).
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V. Cornuaulta, F. Buffetto
b, K.J.D. Lee
a, M.-J. Crépeau
b, M.-C. Ralet
b, J.P. Knox
a (
aU.Leeds,
bINRA). Isolation of RG-I oligosaccharides for monoclonal antibody production and antibody use as detection tools for novel chromatographic analyses of RG-I diversity. Presented at the Gordon conference on Plant Cell Wall Research on August 5-10, 2012 in Waterville (USA) - Poster not publicly available.
F.Buffettoa,
V. Cornuaultb, M.-J. Crépeau
a, O. Tranquet
a, P. Knox
b, F. Guillon
a, M.-C. Ralet
a (
aINRA,
bU.Leeds). Production of low branched rhamnogalacturonan I oligosaccharides from sugar beet pulp. Presented at the Summer Course Glycoscience on June 3-7, 2012 in Gorningen (NL) and at the Plant and Seaweed Polysaccharides Symposium on July 17-20, 2012 in Nantes (FR).
Download Production of low branched rhamnogalacturonan I oligosaccharides from sugar beet pulp
F.Buffettoa,
V. Cornuaultb, M.-J. Crépeau
a, O. Tranquet
a, P. Knox
b, F. Guillon
a, M.-C. Ralet
a (
aINRA,
bU.Leeds). Production of low branched rhamnogalacturonan I oligosaccharides from sugar beet pulp. Presented at the Summer Course Glycoscience on June 3-7, 2012 in Gorningen (NL) and at the Plant and Seaweed Polysaccharides Symposium on July 17-20, 2012 in Nantes (FR).
Download Production of low branched rhamnogalacturonan I oligosaccharides from sugar beet pulp