PhD project: Structure and function of novel Carbohydrate-Active Enzymes (CAZymes) and Carbohydrate Binding Modules (CBMs) involved in plant cell wall degradation

Research interests: Molecular Biology, biochemistry, protein-carbohydrate interactions, carbohydrate active enzymes, carbohydrate binding modules, protein structure.

Organisation: University of Lisbon

Contact:immacolata.venditto(at)yahoo.it

LinkedIn account: www.linkedin.com/pub/immacolata-venditto

Thesis

Structure and Function of Novel Carbohydrate-Active Enzymes (CAZymes) and Carbohydrate-Binding Modules (CBMs) involved in Plant Cell Wall degradation. Defended on 16 January 2015.

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Scientific papers in peer-reviewed journals

A.J. Pires^a, T. Ribeiro^a, A. Thompson^b, I. Venditto^a, V.O. Fernandes^a,c, P. Buke^a, H. Santos^a, V.D. Alves^a, V. Pires^a, L.M.A. Ferreira^a, C.M.G.A. Fontes^a, S. Najmudin^a (^aU.lisbon, ^bSynchrotron Soleil, ^cNZYTech). Purification and crystallographic studies of a putative carbohydrate-binding module from the Ruminococcus flavefaciens FD-1 endoglucanase Cel5A. Acta Christallographica Section F-Structural Biology Communications, Vol. 71, pp. 958-961, part 8 (2015)

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I. Venditto^a, S. Najmudin^a, A.S. Luís^b, L.M.A. Ferreira^a, K. Sakka^c, J.P. Knox^d, H.J. Gilbert^b, and C.M.G.A. Fontes^a (^aU.Lisbon, ^bU.Newcastle, ^cU.Mie, ^dU.Leeds). Family 46 Carbohydrate-Binding Modules contribute to the enzymatic hydrolysis of xyloglucan and β-1,3-1,4-glucans through distinct mechanisms. The Journal Of Biological Chemistry Vol.290, NO.8 (2015)

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I. Venditto^a, A. Goyal^b, A. Thompson^c, L.M.A. Ferreira^a, C.M.G.A. Fontes^a and S. Najmudin^a (^aU.Lisbon, ^bIndian Institute of Technology Guwahati, ^cSynchrotron SOLEIL).Crystallization and preliminary crystallographic studies of a novel noncatalytic carbohydrate- binding module from the Ruminococcus flavefaciens cellulosome. Acta Crystallographica Section F. F70, 45-48 (2015)

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I. Venditto, M.S.J. Centeno, L.M.A. Ferreira, C.M.G.A. Fontes and S. Najmudin(U.Lisbon).Expression, purification and crystallization of a novel carbohydrate-binding module from the Ruminococcus flavefaciens cellulosome. Acta Cryst. F70, 1653-1656 (2014)

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I. Venditto^a, H. Santos^a, J.Sandy^b, J. Sanchez‐Weatherby^b, L.M.A. Ferreira^a, K. Sakka^c, C.M.G.A. Fontes^a and S. Najmudin^a (^aU.Lisbon, ^bDiamond Light Source, ^cU.Mie).Crystallization and preliminary X-ray diffraction analysis of a trimodular endo-β-1,4-glucanase (Cel5B) from Bacillus halodurans. Acta Crystallographica Section F. F70, 1628-1630 (2014)

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I. Venditto^a, H. Santos^a, L.M.A. Ferreira^a, K. Sakka^b, C.M.G.A. Fontes^a and S. Najmudin^a (^aU.Lisbon, ^bU.Mie). Overproduction, purification, crystallization and preliminary X-ray characterization of the family 46 carbohydrate-binding module (CBM46) of endo-β-1,4-glucanase B (CelB) from Bacillus halodurans. Acta Crystallographica Section F. F70, 754-757 (2014)

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A.K. Verma, A. Goyal, F. Freire, P. Bule, I. Venditto, J.L.A. Brás, H. Santos, V. Cardoso, C. Bonifácio, A. Thompson, M.J. Romão, J.A.M. Prates, L.M.A. Ferreira, C.M.G.A.Fontes and Shabir Najmudin (U.Lisbon). Overexpression, crystallization and preliminary X-ray crystallographic analysis of glucuronoxylan xylanohydrolase (Xyn30A) from Clostridium thermocellum. Acta Cryst. F, Vol.69, issue 12, pp 1440-1442 (2013)

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A.S. Luís^a, I. Venditto^a, M.J. Temple^b, A.Rogowski^b, A.Baslé^b, J.Xue^c, J.P. Knox^c, J.A.M. Prates^a, L.M.A. Ferreira^a, C.M.G.A. Fontes^a, S. Najmudin^a, and H.J. Gilbert^b (^aU.Lisbon, ^bU.Newcastle, ^cU.Leeds). Understanding How Noncatalytic Carbohydrate Binding Modules Can Display Specificity for Xyloglucan. The Journal Of Biological Chemistry Vol.288,NO.7,pp.4799–4809 (2013)

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I. Venditto^a, A. Baslé^b, A.S. Luís^a, M.J. Temple^b, L.M.A. Ferreira^a, C.M.G.A. Fontes^a, H.J. Gilbert^b and S. Najmudin^a(^aU.Lisbon, ^bU.Newcastle). Overproduction, purification, crystallization and preliminary X-ray characterization of the C-terminal family 65 carbohydrate-binding module (CBM65B) of endoglucanase Cel5A from Eubacterium cellulosolvens. Acta Crystallographica Section F. F69, 191–194 (2013)

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Oral presentations

I. Venditto^a, S. Najmudin^a , V. Fernandes^a , B. Henrissat^b, L.M.A. Ferreira^a, M. Gro Rydahl^c, W.G.T. Willats^c, H. J. Gilbert^d  and C.M.G.A. Fontes^a (^aU. Lisbon, ^bCNRS, ^cU.Copenhagen, ^dU.Newcastle). Structure and function of novel CAZymes and CBMs involved in plant cell wall degradation. Presented at WallTraC symposium in May 2014 in Paris (FR)

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Posters

I. Venditto^a, V. Fernandes^a, M. Gro Rydahl^b, A. Goyal^c, M.S.J. Centeno^a, L.M.A. Ferreira^a, S. Najmudin^a, W.G.T Willats^b, H.J. Gilbert^d, and C.M.G.A. Fontes^a (^aU.Lisbon, ^bU.Copenhagen, ^c Indian Institute of Technology Guwahati, ^dU.Newcastle). Discovering novel CBMs families in Ruminococcus flavefaciens Cellulosome. Presented at the 5th International Conference on Biosynthesis of Plant Cell Wall on July, 27-31, 2014 in Palm Cove (AU)

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I. Venditto^a, A.S. Luis^b, A. Goyal^c, L.M.A. Ferreira^a, S. Najmudin^a, H.J. Gilbert^b, C.M.G.A. Fontes^a and  (^aU.Lisbon, ^bU.Newcastle, ^cIndian Institute of Technology Guwahati). Structure and mechanism of the endo‐β-1,4-glucanase B (CelB) from Bacillus halodurans. Presented at the 5th International Conference on Biosynthesis of Plant Cell Wall on July, 27-31, 2014 in Palm Cove (AU)

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I. Venditto^a, V. Fernandes^a, M. Gro Rydahl^b, A. Goyal^c, M.S.J. Centeno^a, L.M.A. Ferreira^a, H.J. Gilbert^d, W.G.T Willats^c, C.M.G.A. Fontes^a and S. Najmudin^a (^aU.Lisbon, ^bU.Copenhagen, ^c Indian Institute of Technology Guwahati, ^dU.Newcastle). Structural and functional characterization of novel Carbohydrate Binding Module families in Ruminococcus flavefaciens cellulosome. Presented at the 3rd ENURS and ESRF-day on April 8, 2014 in Lisbon (PT)

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I. Venditto^a, H. Santos^a, A. Goyal^b, L.M.A. Ferreira^a, K.Sakka^c, H.J. Gilbert^d, C.M.G.A. Fontes^a and S. Najmudin^a (^aU.Lisbon, ^bIndian Institute of Technology Guwahati, ^cU.Mie, ^dU.Newcastle). Structure and characterization of the family 46 carbohydrate‐binding module (CBM46) of the endo‐β-1,4-glucanase B (CelB) from Bacillus halodurans. Presented at the 3rd ENURS and ESRF-day on April 8, 2014 in Lisbon (PT)

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I. Venditto^a, A.S. Luís^a, J.A.M. Prates^a, L.M.A. Ferreira^a, M.J. Temple^b, A. Rogowski^b, A. Baslé^b, J. Xue^c, J.P. Knox^c, S. Najmudin^a, C.M.G.A. Fontes^a and H.J. Gilbert^b (^aU.Lisbon, ^bU.Newcastle, ^cU.Leeds). The carbohydrate binding modules family 65 (CBM65) of endoglucanase Cel5A from Eubacterium cellulosolvens display specificity for xyloglucan. Presented at the 2nd ENURS and ESRF-day on February 14-15, 2013 in Lisbon (PT); and at the 10th Carbohydrate Bioengineering Meeting on April, 21-24, 2013 in Prague (CZ).

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